Professor Susanne Mandrup:
| Professor Susanne Mandrup
University of Southern Denmark
Research profile in short
The research in the Mandrup group is focused on understanding the cross-talk between lipid metabolism and transcriptional regulation of gene expression. We are particularly interested in the function, regulation and mechanism of action of the peroxisome proliferator activated receptors (PPARs). To investigate these aspects of the PPARs, we are using a combination of adenoviral, retroviral and lentiviral delivery techniques, chromatin immunoprecipitation (ChIP) and knockdown technologies. S. Mandrup is the coordinator of X-TRA-NET (http://rxrnet.dk/), an FP6 STREP aiming at deciphering the retinoid X receptor (RXR) network using ChIP-on-chip. RXR is the common heterodimerization partner of PPARs and a large number of other nuclear receptors activated by metabolites. We are investigating various aspects of the RXR transcriptional network, including partner-, subtype-, agonist- and response element selectivity.
The laboratory also has a strong interest in the cross-talk between nutrition and gene expression with a focus on pancreatic b -cells and adipocytes. In pancreatic b -cells we are primarily interested in transcriptional regulation of lipid metabolism and the role in b -cell (dys)function. Our research is focused on understanding the mechanisms by which fatty acids and glucose regulate the activity of central transcription factors in glucose and lipid metabolism in pancreatic b -cells and the molecular mechanisms by which these transcription factors subsequently alter gene expression. In adipocytes we investigate the molecular mechanisms underlying the isomer specific delipidating effects of conjugated fatty acids on the function of human adipocytes. Finally, the Mandrup laboratory has a long-standing interest in lipid binding proteins and has generated conventional as well as tissue specific targeted disruption of the acyl-CoA binding protein (ACBP) in mice.
- General molecular biology techniques for cloning, and DNA-, RNA and protein work.
- Cell culture including metabolic studies and transient transfection.
- Isolation of primary human preadipocytes and differentiation in vitro.
- Retroviral, adenoviral, lentiviral transduction of mammalian cells.
- ChIP and ChIP-on-chip
- RNAi-mediated knockdown of gene expression using virally expressed shRNA
- Gene targeting in embryonic stem cells and transgenic technologies
Susanne Mandrup , will contribute to the program by investigating the molecular mechanisms by which marine food components affect cellular signaling and regulation of gene expression. This will include in particular the effects of marine fatty acids on nuclear receptor signaling at single gene level as well as at the genome-wide level. The role of specific receptors, co-factors, kinases etc. in executing the effects of the marine food components will be further investigated using gain-of-function as well as loss-of-function experiments.